| 质粒名称: | SPLICS Mt-ER Short P2A |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 164108 |
| 存储实验室: | Marisa Brini, Tito Calì |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Clontech |
| 空载体大小: | 6532bp |
| 完整质粒大小: | 6532bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Split GFP Mt-ER Short P2A |
| 物种来源: | H. sapiens (human) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Unknown (unknown if destroyed) |
| 3’克隆位点: | Unknown (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | SPLICS Mt-ER Short P2A was a gift from Marisa Brini & Tito Calì (Addgene plasmid # 164108 ; http://n2t.net/addgene:164108 ; RRID:Addgene_164108) |
| 参考文献部分: | An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo. Vallese F, Catoni C, Cieri D, Barazzuol L, Ramirez O, Calore V, Bonora M, Giamogante F, Pinton P, Brini M, Cali T. Nat Commun. 2020 Nov 27;11(1):6069. doi: 10.1038/s41467-020-19892-6. 10.1038/s41467-020-19892-6 PubMed 33247103 |
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SPLICS Mt-ER Short P2A载体质粒基本信息
SPLICS Mt-ER Short P2A质粒图谱载体图谱和SPLICS Mt-ER Short P2A载体序列质粒序列多克隆位点信息
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SPLICS Mt-ER Short P2A质粒序列载体序列
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SPLICS Mt-ER Short P2A质粒载体应用举例
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